<tr>
    <th class="details_info_th">&#20307;&#22806;&#30740;&#31350;<br/>(<span class="font_italic">In Vitro</span>)</th>
    <td class="details_info_td">
        <div>


                <p>2OH-BNPP1 (0.1-50 &#956;M) abrogates TGF&#946; signaling in a dose dependent manner. 2OH-BNPP1 dose-dependently impairs the TGF&#946;-induced phosphorylation of proteins that mediate canonical and non-canonical TGF&#946; signaling in multiple <a href="/ResearchArea/Cancer.html" target="_blank" style="color: #6a4b92; font-weight:bold;">cancer</a> cell lines and normal cell lines<sup><a href="javascript:goCoordinate('cpd_References1');">[1]</a></sup>.</p>
                <p><b>MCE has not independently confirmed the accuracy of these methods. They are for reference only.</b></p>


                <!-- ko if: $root.targetAntProduct() != null && $root.targetAntProduct().length > 0  -->
                <div class="pro-antibodies-wrapper">
                    <div class="pro-related-antibodies-tit"><h3>2OH-BNPP1 &#30456;&#20851;&#25239;&#20307;:</h3></div>
                    <div class="pro-related-antibodies-cont">
                        <ul class="pro-antibodies-cont-list">
                            <!-- ko foreach: $root.targetAntProduct -->
                            <li class="pro-antibodies-cont-item"><a class="" data-bind="attr: {href: '/antibody/' + storeAt + '.html'}, html: name" target="_blank"/></li>
                            <!-- /ko -->
                        </ul>
                        <div class="pro-view-more">
                            <i class="icon-pro-arrow-down"/>
                        </div>
                    </div>
                </div>
                <!-- /ko -->



        </div>
    </td>
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PS D:\python_project\cxsw_spider> python -u "d:\python_project\cxsw_spider\website\mce\req.py"
https://www.medchemexpress.cn/2OH-BNPP1.html
<tr>
    <th class="details_info_th">&#29983;&#29289;&#27963;&#24615;</th>
    <td class="details_info_td">
        <div>
            <p>



                                                    2OH-BNPP1 is an inhibitor of <b>BUB1 kinase</b>, a Ser/Thr kinase, used for the treatment of <a href="/ResearchArea/Cancer.html" target="_blank" style="color: #6a4b92; font-weight:bold;">cancer</a>.



                                            </p>
        </div>
    </td>
</tr>





<tr>
    <th class="details_info_th">&#20307;&#22806;&#30740;&#31350;<br/>(<span class="font_italic">In Vitro</span>)</th>
    <td class="details_info_td">
        <div>


                <p>2OH-BNPP1 (0.1-50 &#956;M) abrogates TGF&#946; signaling in a dose dependent manner. 2OH-BNPP1 dose-dependently impairs the TGF&#946;-induced phosphorylation of proteins that mediate canonical and non-canonical TGF&#946; signaling in multiple <a href="/ResearchArea/Cancer.html" target="_blank" style="color: #6a4b92; font-weight:bold;">cancer</a> cell lines and normal cell lines<sup><a href="javascript:goCoordinate('cpd_References1');">[1]</a></sup>.</p>
                <p><b>MCE has not independently confirmed the accuracy of these methods. They are for reference only.</b></p>


                <!-- ko if: $root.targetAntProduct() != null && $root.targetAntProduct().length > 0  -->
                <div class="pro-antibodies-wrapper">
                    <div class="pro-related-antibodies-tit"><h3>2OH-BNPP1 &#30456;&#20851;&#25239;&#20307;:</h3></div>
                    <div class="pro-related-antibodies-cont">
                        <ul class="pro-antibodies-cont-list">
                            <!-- ko foreach: $root.targetAntProduct -->
                            <li class="pro-antibodies-cont-item"><a class="" data-bind="attr: {href: '/antibody/' + storeAt + '.html'}, html: name" target="_blank"/></li>
                            <!-- /ko -->
                        </ul>
                        <div class="pro-view-more">
                            <i class="icon-pro-arrow-down"/>
                        </div>
                    </div>
                </div>
                <!-- /ko -->



        </div>
    </td>
</tr>



<tr>
    <th class="details_info_th">&#20307;&#20869;&#30740;&#31350;<br/>(<span class="font_italic">In Vivo</span>)</th>
    <td class="details_info_td">
        <div>


                <p>2OH-BNPP1 (50 mg/kg) blocks TGF&#946; signaling in vivo, and significantly decreases abundance of phosphorylated <a href="/proteins/smad2.html" target="_blank" style="color: #6a4b92; font-weight:bold;">SMAD2</a> in tumor-bearing mice<sup><a href="javascript:goCoordinate('cpd_References1');">[1]</a></sup>.</p>
                <p><b>MCE has not independently confirmed the accuracy of these methods. They are for reference only.</b></p>


        </div>
    </td>
</tr>



<tr>
    <th class="details_info_th">&#20998;&#23376;&#37327;</th>
    <td class="details_info_td">
        <div>
            <p id="molecularWeight">

                    297.35


            </p>
        </div>
    </td>
</tr>









<tr>
    <th class="details_info_th">Formula</th>
    <td class="details_info_td">
        <div><p>C<sub>16</sub>H<sub>19</sub>N<sub>5</sub>O</p></div>
    </td>
</tr>


<tr>
    <th class="details_info_th">CAS &#21495;</th>
    <td class="details_info_td">
        <div><p>
            <a href="/cas/833481-73-5.html" class="a-purple fwb" target="_blank">
                833481-73-5
            </a>
        </p></div>
    </td>
</tr>



<tr>
    <th class="details_info_th">&#24615;&#29366;</th>
    <td class="details_info_td">
        <div>
            <p>
                &#22266;&#20307;
            </p>
        </div>
    </td>
</tr>


<tr>
    <th class="details_info_th">&#39068;&#33394;</th>
    <td class="details_info_td">
        <div>
            <p>
                White to off-white
            </p>
        </div>
    </td>
</tr>













<tr>
    <th class="details_info_th">&#36816;&#36755;&#26465;&#20214;</th>
    <td class="details_info_td"><div>


            <p>Room temperature in continental US; may vary elsewhere.</p>


    </div></td>
</tr>


<tr>
<th class="details_info_th">&#20648;&#23384;&#26041;&#24335;</th>
<td class="details_info_td">
    <div id="details_storage">


            <table>

                    <tr>
                        <td style="width: 100px">Powder</td>
                        <th style="width: 50px">-20&#176;C</th>
                        <td>3 years</td>
                    </tr>

                    <tr>
                        <td style="width: 100px"/>
                        <th style="width: 50px">4&#176;C</th>
                        <td>2 years</td>
                    </tr>

                    <tr>
                        <td style="width: 100px">In solvent</td>
                        <th style="width: 50px">-80&#176;C</th>
                        <td>2 years</td>
                    </tr>

                    <tr>
                        <td style="width: 100px"/>
                        <th style="width: 50px">-20&#176;C</th>
                        <td>1 year</td>
                    </tr>

            </table>


    </div>
</td>
</tr>

<tr>
    <th class="details_info_th">Kinase Assay
    <br/><sup><a href="javascript:goCoordinate('cpd_References2');">[1]</a></sup></th>
    <td class="details_info_td">
    <div><p>In vitro kinase assays are performed using 200 ng of protein (BUB1-WT, or a kinase inactive mutant BUB1-KD), and 2 &#181;g of substrate (TGFBRI-WT, TGFBRI-KD, SMAD3 or H2A) in 1X kinase buffer (50 mM Tris-HCl, 150 mM NaCl, 10 mM MgCl<sub>2</sub>, 1% (v/v) Glycerol, 0.1% (v/v) Triton X 100, DTT, PMSF, Na<sub>3</sub>VO<sub>4</sub> (1 mM each), 2 mM NaF and &#946;-glycerol phosphate) in 20 &#181;L volume containing 10 &#181;Ci <sup>32</sup>p-ATP and 300 &#181;M cold ATP. Reactions are run at 30&#186;C for 0.5-1 hour and quenched using Laemmli buffer and resolved using a 4-12% Bis-Tris gel. Quantitative autoradiography is performed using a Typhoon FLA 9000 scanner.</p>
        <p><b>MCE has not independently confirmed the accuracy of these methods. They are for reference only.</b></p>
    </div>
    </td>
</tr>



<tr>
    <th class="details_info_th">Animal Administration
    <br/><sup><a href="javascript:goCoordinate('cpd_References2');">[1]</a></sup>
    </th>
    <td class="details_info_td">
        <div><!--<p>2OH-BNPP1 is dissolved in DMSO.</p>-->
        <p>Cell line-derived xenografts are established by implanting 2.5&#215;10<sup>6</sup> A549 cells subcutaneously into each flank of 4-6 week old male SCID mice. When tumors reach a volume between 40 to 60 mm<sup>3</sup>, mice are injected with a single intraperetoneal dose of SB431542 (10 mg/kg body weight), a dose previously reported to inhibit TGF&#946; signaling in mouse models, or 2OH-BNPP1 (50 mg/kg), or vehicle (DMSO). Tumors are excised 4 hours after treatment and fixed in formalin. Paraffin-embedded sections are stained using an antibody for phosphorylated SMAD2, and micrographs are taken with an Olympus microscope fitted with an Olympus DP-70 high resolution digital camera. The proportion of nuclei that stains positive for phosphorylated SMAD2 are counted in three random fields per tumor per treatment condition [vehicle (n=4), SB431542 (n=2), and 2OH-BNPP1 (n=5)]. A two -ided Student's t-test is performed to assess statistical significance. Slides are adjusted for brightness and contrast with Adobe Photoshop CS2, but the micrographs undergo no further manipulations.</p>
        <p><b>MCE has not independently confirmed the accuracy of these methods. They are for reference only.</b></p>
        </div>
    </td>
</tr>


<tr>
    <th class="details_info_th">&#21442;&#32771;&#25991;&#29486;</th>
    <td class="details_info_td" id="cpd_References2">
        <div>
            <ul class="pro_info_ref">
                    <li>

                    <p>[1]. <a href="https://www.ncbi.nlm.nih.gov/pubmed/25564677" rel="nofollow" target="_blank">Nyati S, et al. The kinase activity of the Ser/Thr kinase BUB1 promotes TGF-&#946; signaling. Sci Signal. 2015 Jan 6;8(358):ra1.</a>
                        &#160;<a href="/mce_publications/25564677.html" target="_blank" style="color: #6a4b92;"><b>[Content Brief]</b></a>
                    </p>

                </li>
            </ul>
        </div>
    </td>
</tr>